Figure 5.

Dynamic clamp reveals that a shift in I_A V_h is sufficient to reproduce the effects of a Cav3–Kv4 interaction. A, Inactivation curves of the I_A model show the difference in I_A availability before and after a left shift corresponding to the measured effect of blocking the Cav3–Kv4 complex in lobules 2 and 9 (see Fig. 2C). The predicted effect on I_A availability for a −80 mV holding potential is shown by dashed lines. B, Examples of I_A predicted to be evoked by a spike response (V) for control conditions and after blocking the Cav3–Kv4 interaction. The net inward difference current (I_diff) to inject through dynamic clamp is shown in the bottom row. C, Recordings of granule cell firing in response to an injected current pulse before and after applying a shift in I_A V_h under dynamic clamp according to the contribution of the Cav3–Kv4 interaction (as in A and B). D, E, Frequency–current plots (D) and bar plots of the gain of firing (E) under control conditions and during dynamic clamp shifts in I_A V_h (as in A and B). *p < 0.05, Student's paired t test.