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. 2014 Jun 18;34(25):8546–8556. doi: 10.1523/JNEUROSCI.5002-13.2014

Figure 3.

Figure 3.

Enhanced microglial activation after repeated treatment with LPS. A, Microglial activation marker CD68 mRNA levels in the whole brain of mice on experimental day 5 or 19 after single or repeated treatment with PBS control or LPS. Cd68 gene transcripts were increased on both experimental days after repeated treatment with LPS. n ≥ 3 mice, n.s., Not significant. ***p < 0.001. B, Immunofluorescence staining of CD68 (red) and Iba1 (green) in SNpr on experimental day 5 or 19 after repeated treatment with PBS control or LPS. CD68 immunoreactivity was increased after repeated LPS application on day 5. Scale bar, 10 μm. Representative images of n ≥ 3 mice are shown. C, Quantification of CD68 immunoreactivity area in the SNpr sections of mice on experimental day 5 and 19 after single or repeated PBS control or LPS treatment. Microglial CD68 immunoreactivity was increased on day 5 after repeated systemic LPS challenge. n ≥ 3 mice. n.s., Not significant. *p < 0.05. **p < 0.01.