Table 2.
Target | Host | Dilution | Source |
---|---|---|---|
Antibodies for IHC | |||
APC (CC1) | Mouse | 1:200 | Abcam |
FASN | Rabbit | 1:200 | |
Ki67 | Rat | 1:25 | DakoCytomation |
P-S6 | Rabbit | 1:200 | Cell Signaling Technology |
Lipin-1 | Rabbit | 1:50 | |
MBP | Rat | 1:200 | AbD Serotec |
NF-160 | Mouse | 1:200 | Sigma |
Olig2 | Rabbit | 1:500 | Millipore |
PDGFRα | Rat | 1:100 | BD Pharmingen |
Tcf-4 | Mouse | 1:100 | Millipore |
Antibodies for WB | |||
P-Akt S473 | Rabbit | 1:1000 | Cell Signaling Technology |
P-Akt T308 | Rabbit | 1:1000 | |
Akt | Rabbit | 1:1000 | |
P-4-EBP1 T37/46 | Rabbit | 1:1000 | |
P-4-EBP1 S65 | Rabbit | 1:1000 | |
4EBP1 | Rabbit | 1:1000 | |
P-Erk1/2 T202/Y204 | Rabbit | 1:1000 | |
Erk1/2 | Mouse | 1:1000 | |
Lipin-1 | Rabbit | 1:1000 | |
mTOR | Rabbit | 1:1000 | |
P-S6 S235/236 | Rabbit | 1:1000 | |
S6 | Rabbit | 1:1000 | |
P-p70 S6K T389 | Rabbit | 1:1000 | |
p70 S6K | Rabbit | 1:1000 | |
TSC1 | Rabbit | 1:1000 | |
TSC2 | Rabbit | 1:1000 | |
Raptor | Rabbit | 1:200 | |
Rictor | Rabbit | 1:200 | |
MBP | Rat | 1:1000 | AbD Serotec |
α-tubulin | Mouse | 1:10000 | Sigma |
PLP | Rabbit | 1:1000 | Abcam |
FASN | Rabbit | 1:1000 | |
Scap isoform 4 | Rabbit | 1:500 | |
SREBP2 | Rabbit | 1:200 | |
MOG | Mouse | 1:500 | Santa Cruz Biotechnology |
SREBP1 K-10 | Rabbit | 1:50 | |
Scap isoform 1 | Goat | 1:200 |
Antibodies to MAG were a generous gift from Dr Steven Scherer (University of Philadelphia, Philadelphia, PA); antibodies to HMGCR and IDI1 were a generous gift from Dr. Werner Kovacs (ETH Zürich, Switzerland).