Table 2.
List of antibodies
| Target | Host | Dilution | Source |
|---|---|---|---|
| Antibodies for IHC | |||
| APC (CC1) | Mouse | 1:200 | Abcam |
| FASN | Rabbit | 1:200 | |
| Ki67 | Rat | 1:25 | DakoCytomation |
| P-S6 | Rabbit | 1:200 | Cell Signaling Technology |
| Lipin-1 | Rabbit | 1:50 | |
| MBP | Rat | 1:200 | AbD Serotec |
| NF-160 | Mouse | 1:200 | Sigma |
| Olig2 | Rabbit | 1:500 | Millipore |
| PDGFRα | Rat | 1:100 | BD Pharmingen |
| Tcf-4 | Mouse | 1:100 | Millipore |
| Antibodies for WB | |||
| P-Akt S473 | Rabbit | 1:1000 | Cell Signaling Technology |
| P-Akt T308 | Rabbit | 1:1000 | |
| Akt | Rabbit | 1:1000 | |
| P-4-EBP1 T37/46 | Rabbit | 1:1000 | |
| P-4-EBP1 S65 | Rabbit | 1:1000 | |
| 4EBP1 | Rabbit | 1:1000 | |
| P-Erk1/2 T202/Y204 | Rabbit | 1:1000 | |
| Erk1/2 | Mouse | 1:1000 | |
| Lipin-1 | Rabbit | 1:1000 | |
| mTOR | Rabbit | 1:1000 | |
| P-S6 S235/236 | Rabbit | 1:1000 | |
| S6 | Rabbit | 1:1000 | |
| P-p70 S6K T389 | Rabbit | 1:1000 | |
| p70 S6K | Rabbit | 1:1000 | |
| TSC1 | Rabbit | 1:1000 | |
| TSC2 | Rabbit | 1:1000 | |
| Raptor | Rabbit | 1:200 | |
| Rictor | Rabbit | 1:200 | |
| MBP | Rat | 1:1000 | AbD Serotec |
| α-tubulin | Mouse | 1:10000 | Sigma |
| PLP | Rabbit | 1:1000 | Abcam |
| FASN | Rabbit | 1:1000 | |
| Scap isoform 4 | Rabbit | 1:500 | |
| SREBP2 | Rabbit | 1:200 | |
| MOG | Mouse | 1:500 | Santa Cruz Biotechnology |
| SREBP1 K-10 | Rabbit | 1:50 | |
| Scap isoform 1 | Goat | 1:200 | |
Antibodies to MAG were a generous gift from Dr Steven Scherer (University of Philadelphia, Philadelphia, PA); antibodies to HMGCR and IDI1 were a generous gift from Dr. Werner Kovacs (ETH Zürich, Switzerland).