Expression of 380-slit-GAL4/UAS-GFPCD8 in midline glia. The 380-slit-GAL4 has been described previously to drive expression in midline glia in embryo and larva of Drosophila. Here, we showed that the 380-slit-GAL4 driver is expressed in midline glia during PD. A, At 7% of PD, midline glia were clearly defined with UAS-GFPCD8 expression. This expression pattern is representative of the 380-slit-GAL4 driver from 7% to 46% of PD. B, At 47% of PD, the glia were clearly visible with GFP expression. This expression pattern is representative of the 380-slit-GAL4 driver from 47% to 75% of PD. Schematic of early pupal CNS, with midline glia shown in green. Scale bar, 20 μm.