Skip to main content
. 2014 Jun 11;34(24):8300–8317. doi: 10.1523/JNEUROSCI.0159-14.2014

Figure 1.

Figure 1.

Comparison of labeling obtained with two fixation protocols. Top, Micrographs of the GABAAR α2 and α3 subunits, gephyrin, of the GlyR α1 subunit and of the GABAAR β3 subunit using a mild fixation protocol with immersion of frozen sections of fresh tissue in 4% PFA for 10 min. Bottom, Same labeling with a standard 4% PFA perfusion followed by 2 h postfixation. Identical antibody concentrations and confocal laser settings were used for images obtained with both fixation protocols. For SN, the number of clusters per 100 μm2 gives an index of the cluster detection for each marker in both fixation protocols. Values are expressed as mean ± SD. Scale bar, 40 μm.