Figure 5.

Minocycline (Mino) treatment reduces photoreceptor apoptosis. The nuclear layers were stained with DAPI (A–C, blue). No TUNEL-positive photoreceptor cells (red) are observed in the ONL at both P16 (D) and P19 (E). TUNEL-positive photoreceptor cells begin to appear in the ONL at P22 (F). G, Quantification of TUNEL-positive photoreceptor cells in the ONL at three time points. Note that the number of TUNEL-positive photoreceptor cells significantly decreased in minocycline-treated rd10 mice at P19 and P22. Results are presented as mean ± SD; **p < 0.01, n = number of retinas in each group. OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar, 20 μm.