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. 2014 Jul 2;34(27):8976–8987. doi: 10.1523/JNEUROSCI.0678-14.2014

Figure 3.

Figure 3.

Exercise prevents the modulation of the expression of the cation chloride cotransporters KCC2 and NKCC1. Western blot analysis showing KCC2 (A) and NKCC1 (B) protein levels in the lumbar spinal cord (L4–L6) of intact, SCI-7d, SCI-28d, and SCI-28d+exercise animals. KCC2 decreased significantly after a complete SCI transection, whereas NKCC1 levels increased. Exercise returned KCC2 and NKCC1 expression levels toward intact. KCC2 and NKCC1 proteins were detected and quantified by Western blots using actin as a standard control (*compared with intact; §compared with SCI-28d+exercise). C, KCC2/NKCC1 ratio is decreased after SCI, but not in exercised animals. D, Regression analysis showing a negative relationship between KCC2 and NKCC1 protein levels in the lumbar enlargement (p = 0.008).