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. 2014 Jul 30;34(31):10311–10324. doi: 10.1523/JNEUROSCI.5128-13.2014

Figure 6.

Figure 6.

BM integrity is improved by unc-52 mutation. A, Double labeling with Phmr-1b::mCherry::RAB-3 (red) and Laminin::GFP (green) in unc-52(e998) mutants. B, Double labeling with Phmr-1b::mCherry::RAB-3 (red) and Laminin::GFP (green) in unc-52(e998);emb-9(xd51) animals. A, B, The Phmr-1b::mCherry::RAB-3 puncta tightly align with Laminin::GFP. C, Quantification of BM integrity based on the number of gaps per 50 μm length of Laminin::GFP. ***p < 0.001. D, Ectopic synaptic boutons labeled by Phmr-1b::GFP::RAB-3 (arrows) in gon-1(q518) animals. E, The ectopic presynaptic bouton defect is greatly suppressed in unc-52(e998);gon-1(q518) mutants compared with gon-1(q518). F, G, The gonad arm underextension defect in gon-1(q518) mutants is not suppressed in unc-52(e998);gon-1(q518) animals. Dashed lines highlight the gonad regions. H, Quantification of ectopic boutons for selected genotypes using the Phmr-1b::GFP::RAB-3 marker. The number of ectopic boutons is significantly lower when gon-1 is doubled with unc-52(e998). Scale bars, 5 μm.