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. 2014 Apr 23;34(17):6084–6097. doi: 10.1523/JNEUROSCI.4261-13.2014

Figure 7.

Figure 7.

Aβo treatment on primary cortical neuron cultures induces synaptic mislocalization of endogenous tau that is disrupted by synaptic activation. A, Confocal imaging of cortical neurons (14 DIV) cotransfected with EGFP-Tau and LifeAct-RFP. Left, Spatial repartition of EGFP-Tau (in green) and LifeAct-RFP (in red) in untreated neurons. Scale bar, 5 μm. Right, Higher magnification of EGFP-Tau and LifeAct-RFP in a dendritic spine (designated box in the left) neuron showing the effect of 15 min of Aβ on tau localization (green) and actin cytoskeleton (red) followed by 15 min of Bic/4-AP. Scale bar, 1 μm. B, Representative Western blots of protein expression from fractionated primary cortical neurons treated with Aβo (100 nm) and then for 15 min with Bic/4-AP. Presynaptic protein (synaptophysin), postsynaptic proteins (PSD-95, GluA1,GluN2A, GluN2B,Fyn), cytoskeleton protein (actin), and tau in PSD-fraction (Triton-insoluble fraction) were analyzed. C, Quantitative analysis of PSD-95, GluA1, GluN2B, GluA1, Fyn, actin, and tau in PSD fraction (mean ± SEM, 2-tailed Student's t test, N ≥ 7). D, Confocal imaging of cortical neurons (14 DIV) transfected with GFP membrane. Top, Untreated neurons. Scale bar, 10 μm. Middle and Right, higher magnification of GFP membrane in dendrites (scale bar, 5 μm) and dendritic spine (designated box in the middle) from a neuron showing the effect of 15 min of Aβ treatment on spines followed by 15 min of Bic/4-AP. Scale bar, 1 μm.