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. 2014 Jul 23;34(30):10010–10021. doi: 10.1523/JNEUROSCI.0934-14.2014

Figure 4.

Figure 4.

Intra-BLA infusion of Sal003 prevented retrieval-related eIF2α dephosphorylation in the BLA and impaired the reconsolidation of morphine-induced CPP. The data are expressed as mean ± SEM. A, Timeline of the experiment. B, CPP scores during baseline preference and tests for the expression of morphine-induced CPP in rats injected with Sal003 (0, 2, 6, and 20 μm/side) in the BLA immediately after exposure to the morphine-paired context on day 10 (n = 8–10 per group). *p < 0.05 compared with CPP Test 1 in the Sal003 group (20 μm/side) and compared with Sal003 (0 μm/side) in CPP Test 2. C, Phosphorylated eIF2α, ATF4, and total eIF2α protein levels and representative Western blots in the BLA after CPP Test 2. The data are expressed as a percentage of phosphorylated eIF2α, ATF4, and total eIF2α in naive control rats (n = 6 per group). In the BLA, Sal003 (20 μm/side) increased phosphorylated eIF2α and ATF4 after CPP Test 2. *p < 0.05 compared with Sal003 (0 μm/side) group. D, CPP scores during baseline preference and tests for the expression of morphine-induced CPP in rats injected with vehicle (0.5 μl/side) or Sal003 (20 μm/side) in the CeA immediately after exposure to the morphine-paired context on day 10 (n = 7–8 per group). E, Timeline of the experiment. F, CPP scores during baseline preference and a test for the expression of morphine-induced CPP in rats decapitated 10 min after exposure to the morphine-paired context on day 10 (n = 5 per group). G, Western blot analysis of phosphorylated and total eIF2α in the BLA in the no-retrieval (NoR)-Vehicle, NoR-Sal003, retrieval (Ret)-Vehicle, and Ret-Sal003 groups. The phosphorylation level of eIF2α decreased in the Ret-Vehicle group (n = 5 per group). *p < 0.05 compared with NoR-Vehicle group.