Figure 2.
Loss of Smarcb1 impairs proliferation and activates Wnt signaling in cerebellar granule cell precursors. Cerebellar granule cell precursors were isolated from Smarcb1fl/fl mice on day P5, cultured, and transduced with IRES-GFP or Cre-IRES-GFP viruses. Thirty-six hours after transduction, cells were sorted for GFP-positive cells and experiments were performed. Cre-IRES-GFP-transduced cells displayed a severe reduction of Smarcb1 expression, as expected (A). Furthermore, proliferation was significantly decreased (B), and cells within the Cre-IRES-GFP condition were characterized by a massive upregulation of Wnt target genes Tcf4, Lef1, Dkk1, and Axin2. No significant changes were observed regarding the expression of Ink4a, Gli1, Gli2, Gli3 or Cyclin D1 (C).