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. 2014 Jul 16;34(29):9574–9589. doi: 10.1523/JNEUROSCI.4302-13.2014

Figure 2.

Figure 2.

Identification of the major SUMO acceptors on CREB. A, Examination of PIAS1 SUMOylation of CREB at Lys109, Lys122, Lys141, Lys271, and Lys278 in HEK293T cells. B, Examination of PIAS1 SUMOylation of CREB at Lys289, Lys290, Lys291, and Lys295 in HEK293T cells. C, Examination of PIAS1 SUMOylation of CREB at Lys309, Lys316, Lys319, and Lys325 in HEK293T cells. D, Confirmation of PIAS1 SUMOylation of CREB at Lys271 and Lys290 in HEK293T cells. The quantified result is shown on the right. E, PIAS1 overexpression increased the expression level of CREB in HEK293T cells. The quantified result over time is shown below. CHX, Cycloheximide. Data are shown as mean ± SEM. F, CREB SUMOylation by PIAS family proteins, other E3 ligases, and E2 ligase in HEK293T cells. The quantified result is shown on the right. ΔFG, Partial sequence of full-length RanBP2. Data are expressed as mean ± SEM. *p < 0.05; #p < 0.001. Experiments were performed in duplicate or triplicate.