Figure 10.

Locomotor activity of ITP/PDF-double-KD flies and controls in LD 12:12. A, Average activity profiles were calculated for each genotype and were normalized to the highest activity value. PDF-KD flies (tim(UAS)G4>dcr2;pdf-RNAi) show the typical advanced E activity and reduced M activity. The same phenotypes can be seen in ITP/PDF-double-KD flies (tim(UAS)G4>dcr2;itp-RNAi/pdf-RNAi). In addition, these flies show a less pronounced siesta compared with the other genotypes. n = number of investigated flies; black areas indicates darkness, gray areas indicates light of 100 lux; black line = mean, gray lines = SEM; T = 20°C. B, Relative activity levels for day (left) and night (right) were calculated as mean beam crosses per minute during the light phase or the dark phase relative to the average of beam crosses during the whole day. Relative daytime and nighttime activities were significantly dependent on the genotype. In particular, ITP/PDF-double-KD flies showed a slight reduction in daytime activity. The tendency toward reduced daytime activity in ITP-KD flies (tim(UAS)G4>dcr2;itp-RNAi) was similar to the results of Figure 4B. Both ITP-KD flies and ITP/PDF-double-KD flies showed a significant increase in nighttime activity. C, Mean E activity (ZT06 to ZT18) was calculated in relation to mean M activity (ZT18 to ZT06) as in Figure 4C and was significantly dependent on the genotype. ITP-KD flies showed significantly less E activity than the controls and PDF-KD flies (cf. also Fig. 4C). E activity in ITP/PDF-double-KD flies was similarly reduced. The control strains in B and C (dark gray bars) are in the following order from left to right: tim(UAS)G4>dcr2, dc2;itp-RNAi, and dcr2;pdf-RNAi. Error bars indicate SEM. n ≥ 21 in all panels. Lowercase letters indicate significant differences (different letters indicate p < 0.05).