Figure 3.

ITP staining intensity in clock neuron cell bodies in CS compared with the clock mutants per⁰¹ and Clk^AR. A, Anti-ITP staining on male adult brains of CS, per⁰¹, and Clk^AR at ZT02 in LD 12:12. B, Quantification of the ITP staining intensity in the fifth sLNv (left) and the LNd (right) in the different genotypes (n = 12). Anti-ITP staining intensity was significantly reduced in both cells in Clk^AR mutants compared with CS and per⁰¹. Error bars indicate SEM. Scale bars, 10 μm. **p < 0.001 in pairwise comparisons.