Figure 9.

ITP and PDF cycling in the dorsal brain terminals in ITP-overexpression flies. A, tim(UAS)G4>ITP² flies lack a significant difference in ITP staining at ZT2 and ZT20 in the PI. B, The difference in PDF-staining intensity between the two time points is reduced in tim(UAS)G4>ITP² flies. C, tim(UAS)G4>ITP² flies show a higher percentage of aberrant PDF-fibers in the PI than the other fly strains. D, Typical brain of a tim(UAS)G4>ITP² fly stained with anti-PDF at ZT2 showing aberrant fibers stemming from the lLNv in the PI (cell bodies not in the picture). *Significant differences between the two time points in each genotype. Scale bar, 10 μm. n ≥ 10 for each time point and genotype.