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. 2014 Jul 16;34(29):9621–9643. doi: 10.1523/JNEUROSCI.3991-13.2014

Figure 1.

Figure 1.

AMPA receptors physically interact specifically with A1Rs. A, GluA2-containing AMPAR immunoprecipitated complexes from rat hippocampus contained A1Rs, and A1R-immunoprecipitated complex contained GluA2 subunits. B, GluA1 immunoprecipitate from rat hippocampal tissue also contained A1Rs, and the A1R immunoprecipitate likewise contained GluA1 subunits. C, Coimmunoprecipitation of GluA2 did not include A2ARs, and A2AR antibody did not immunopreciptitate GluA2-containing AMPARs. D, GluA1 immunoprecipitate did not contain A2ARs, and A2AR immunoprecipitate did not contain GluA1 subunits. These forward and reverse coimmunoprecipitation studies are from at least three independent experiments, using hippocampal brain lysates from P18–P28 d rats. The molecular weights of the specific bands on the blots were estimated from prestained protein standards and are as follows: A1R (37 kDa), A2AR (45 kDa), GluA1 (106 kDa), and GluA2 (102 kDa). Amount of protein loaded into gels, antibody sources, and dilutions are indicated in the Materials and Methods.