NE stimulates GnIH release and decreases plasma LH concentration. A, Effects of NE administration on the release of GnIH from diencephalic explants. Diencephalic tissue explants were treated with the indicated concentrations of NE for 60 min. One-way ANOVA followed by Tukey's multiple comparisons test, *p < 0.05 versus vehicle. B, Effects of intracerebroventricular injection of NE (10 ng, 100 ng, or 1 μg of NE or vehicle) on plasma LH level. One-way ANOVA followed by Dunnett's multiple comparisons test, *p < 0.05 versus vehicle.