Table 1.
Primers used for real-time PCR and to make in situ hybridization probe
| Gene | Primer sequences | GenBank accession number |
|---|---|---|
| Real-time PCR | ||
| Quail GnIH | AB820136 | |
| Forward | 5′-ATGGTGCGTGCCTAGATGAAC-3′ | |
| Reverse | 5′-AGCAACTGAATTTGGCACTTTG-3′ | |
| Quail GnRH-I | AB820137 | |
| Forward | 5′-GCTTGGCTCAACACTGGTCTT-3′ | |
| Reverse | 5′-GGGCATTCAGCCTCCTTCA-3′ | |
| Quail 18S rRNA | AF173611 | |
| Forward | 5′-TGATTAAGTCCCTGCCCTTT-3′ | |
| Reverse | 5′-CCGAGGACCTCACTAAACCA-3′ | |
| In situ hybridization probe | ||
| Quail ADRα2A | AB820133 | |
| Forward | 5′-CCACGTCCTGGTCATCATTG-3′ | |
| Reverse | 5′-ATGACGAAGACCCCAATCAC-3′ | |
| Quail ADRα2C | AB820134 | |
| Forward | 5′-CTCTGGTCATGCCTTTCTCC-3′ | |
| Reverse | 5′-TCCCGGCAAATACCATAGAG-3′ |