Figure 6.

cPKC positively regulates K⁺-stimulated DA release. A, cPKCs and βPIX are expressed in PC12 cells. PC12 cell lysates were analyzed with immunoblotting with anti-PKCα, β1, β2, γ, and βPIX antibodies. B, DA release in PC12 cells was measured with various K⁺ concentrations. The results are expressed as mean ± SEM (n = 3). C, Amounts of DA release that were stimulated in response to 100 mm K⁺ with 1 μm GFX or 1 μm Gö6976 were measured in PC12 cells (n = 3; *p < 0.05, unpaired t test). DA release levels were normalized to the levels released in response to 100 mm K⁺, which were set to 100%. The results are expressed as mean ± SEM (n = 3–5). D, E, Uptake of ³H-DA into PC12 cells with or without GFX and Gö6976 were measured. The results are expressed as mean ± SEM (n = 3). F, TPA stimulated DA release in PC12 cells. DA release in PC12 cells was measured with 1 μm TPA in the absence or presence of 1 μm GFX. DA release levels were normalized to that of 1 μm TPA stimulation, which was set to 100%. The results are expressed as mean ± SEM (n = 3, *p < 0.01, unpaired t test).