Table 2.
List of phosphopeptides with a PKC phosphorylation motif
| Protein name | Accession no. | Sequence | Site | No. of phosphopeptides |
Lowest peptide score |
Highest peptide score |
Average ion intensity |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | KO | WT | KO | WT | KO | WT | KO | WT/KO | ||||
| Gap junction alpha-1 protein | NP 034418 | KVAAGHELQPLAIVDQRPSSRA | S365 | 7 | 0 | 11 | − | 28 | − | 3 | 0.4 | 7.3 |
| Disks large-associated protein 1 | NP 808307 | RSLDSLDPAGLLTSPKF | S437 | 5 | 0 | 48 | − | 77 | − | 3.4 | 0.8 | 4.4 |
| MAP kinase-activating death domain protein | NP 001171190 | RATLSDSEIETNSATSAIFGKA | T1235 | 8 | 4 | 40 | 26 | 90 | 58 | 3.7 | 1 | 3.9 |
| DnaJ homolog subfamily C member 5 | NP 001258513 | RSLSTSGESLYHVLGLDKN | S10 | 54 | 55 | 3 | 4 | 88 | 85 | 969 | 299.5 | 3.2 |
| Calnexin | NP 001103969 | KAEEDEILNRSPRN | S582 | 6 | 2 | 25 | 22 | 31 | 34 | 3.1 | 1.8 | 1.8 |
| Stathmin | NP 062615 | KRASGQAFELILSPRS | S16 | 26 | 23 | 8 | 6 | 93 | 84 | 12.1 | 7.5 | 1.6 |
| Stathmin | NP 062615 | RASGQAFELILSPRS | S25 | 25 | 17 | 5 | 19 | 81 | 83 | 7.4 | 5.9 | 1.3 |
| Rho guanine nucleotide exchange factor 7 | NP 001106989 | RMSGFIYQGKL | S340 | 3 | 0 | 24 | − | 59 | − | 1.8 | 1.4 | 1.4 |
| Reticulon-4 | NP 077188 | RRGSGSVDETLFALPAASEPVIPSSAEKI | S165 | 15 | 8 | 34 | 41 | 70 | 59 | 2.3 | 1.7 | 1.3 |
| α-adducin | NP 001019629 | KFRTPSFLKK | S724 | 3 | 2 | 7 | 9 | 29 | 25 | 6.1 | 4.8 | 1.3 |
| β-adducin | NP 001258786 | KDIATEKPGSPVKS | S594 | 10 | 8 | 10 | 17 | 50 | 56 | 0.4 | 0.3 | 1.2 |
Phosphopeptides with a WT/KO ratio >1.0 are listed. The numbers of phosphorylation sites are based on the results of mice in PhosphoSitePlus (www.phosphosite.org). S and T are the identified phosphorylation sites. Number of phosphopeptides means the total numbers of the phosphopeptides in each group (n = 4). Highest or lowest peptide score means the highest or lowest peptide score in each group (n = 4). Peptides that were not detected are shown as −.