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. 2014 Nov 5;34(45):14995–15008. doi: 10.1523/JNEUROSCI.2228-14.2014

Figure 1.

Figure 1.

BDNF increased Girdin S1416 phosphorylation through the TrkB/PI3-K/Akt pathway in cultured mouse hippocampal neurons (DIV 14). A, B, Time-course changes in the levels of phosphorylated Akt (A) and Girdin (B) after BDNF (50 ng/ml) application in cultured E15–E16 mouse hippocampal neurons (DIV 14). Data are expressed as mean ± SEM (n = 5 at each time point). One-way ANOVA, F(3,16) = 16.107, p < 0.01 for A; F(3,16) = 4.412, p < 0.05 for B. *p < 0.05 and **p < 0.01 compared with the control (Tukey–Kramer post hoc test). C–E, Effects of Trk (C), PI3-K (D), and Akt (E) inhibitors on BDNF-induced Girdin phosphorylation in cultured E15–E16 mouse hippocampal neurons (DIV 14). Neurons were stimulated with BDNF (50 ng/ml) for 15 min in the presence of DMSO (1%), K252a (0.2 μm, 0.6 μm, or 1.2 μm), LY294002 (10 μm, 30 μm, or 50 μm), and Akt inhibitor IV (0.1 μm, 0.5 μm, or 1.0 μm). Data are expressed as mean ± SEM (n = 4 in each group). DMSO was treated as a control of each inhibitor (shown as 0 μm). One-way ANOVA, F(4,15) = 3.885, p < 0.05 for C; F(4,15) = 8.967, p < 0.01 for D; F(3,16) = 12.837, p < 0.01 for E. *p < 0.05 and **p < 0.01 compared with the control (Tukey–Kramer post hoc test). #p < 0.05 and ##p < 0.01 compared with the BDNF-treated control (Tukey–Kramer post hoc test).