Increased phosphorylation of Akt, Girdin S1416, and NR2B of NMDA receptor in the hippocampus of mouse after fear conditioning. A–C, Western blot analyses showing the time-course changes in the phosphorylated levels of Akt S473 (A), Girdin S1416 (B), and NR2B Y1472 (C) in the DG. The DG of 8- to 9-week-old mice were removed 5 min, 15 min, or 30 min after fear conditioning. Control mice were placed in a conditioning chamber without shock and tone. Data are expressed as mean ± SEM (n = 5 mice per time point). One-way ANOVA; F(3,16) = 4.795, p < 0.05 for A; F(3,16) = 4.909, p < 0.05 for B; F(3,16) = 4.120, p < 0.05 for C. *p < 0.05 compared with control mice (Tukey–Kramer post hoc test).