Figure 1.
Similar expression of human α-synuclein in SN DA and VTA DA neurons. A, B, Confocal images at high-magnification (60× oil) showing TH-positive signal (red) and human α-synuclein-positive signal (a-synh, green) in SN and VTA midbrain regions of control (A) and A53T-SNCA mice (B), respectively. Scale bar, 20 μm. C, Histogram showing the distributions of human α-synuclein immunosignal intensities, measured for TH-colocalized ROIs, in DA SN (red) and DA VTA (black) neurons of A53T-SNCA mice. D, Comparison of the mean ± SEM a-synh immunosignal (TH-colocalized ROIs) from DA SN and DA VTA neurons in control and A53T-SNCA mice, respectively. ns, Not significant; Mann–Whitney test.