Figure 7.

Increased Kv4.3 channel expression in the plasma membrane of DA SN neurons in A53T-SNCA mice. A, Electron micrographs showing immunoreactivity for Kv4.3 in the SN. Pre-embedding (top) and SDS-FRL replica (bottom) immunoelectron microscopy demonstrate that the Kv4.3 proteins (IG particles) are distributed in a clustered fashion along the extrasynaptic plasma membrane of dendritic shafts (Den) of putative dopaminergic cells on tissues obtained from control (control; top and bottom left; n = 36 dendrites) and transgenic (A53T-SNCA; top and bottom right; n = 46 dendrites) mice. Both immunocytochemical methods revealed higher surface expression of the channel subunit in transgenic animals than in control animals. B, This was further validated by quantification of IG density for Kv4.3 on replicas, which was significantly lower in neurons from WT compared with cells from A53T-SNCA mice (t test; **p = 0.0045, *p = 0.0142). P-face, Protoplasmic face; E-face, exoplasmic face; b, bouton; cf, cross-fractured. Scale bars, 0.2 μm.