Table 1.
Antibody (anti) | Manufacturer and catalog no. | Marker of: | Working dilution |
---|---|---|---|
BrdU | Abcys, ABC 117-7513 | Proliferating cells | 1:1000 |
Megabase Research Products | 1:2000 | ||
Calb | Swant, 300 | Calbindin-interneurons | 1:2000 |
active casp3 | Cell Signaling, asp175 | Apoptotic cells | 1:300 |
CD68 | Serotec, MCA1957GA | Activated microglia | 0.5 μg/ml |
DCX | Abcam, ab 18723 | Neuroblasts | 0.5 μg/ml |
GFAP | Dako, 20334 | Astrocytes | 4 μg/ml |
Iba1 | Wako Chemicals, 016-20001 | Microglia | 1 μg/ml |
NeuN | Millipore Bioscience Research Reagents, MAB377 | Neurons | 5 μg/ml |
Olig2 | Santa Cruz Biotechnology, sc48817 | Oligodendrocyte progenitors | 1:500 |
Pax6 | Millipore Bioscience Research Reagents, AB2237 | Dopaminergic neurons | 1:700 |
TH | ImmunoStar, 22941 | Dopaminergic neurons | 1:4000 |