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. 2014 Oct 29;34(44):14624–14632. doi: 10.1523/JNEUROSCI.2117-14.2014

Figure 1.

Figure 1.

PERK is the major kinase to determine basal levels of p-eIF2α. A, Schematic description of the plasmids used to generate lentiviruses. B, Immunoblot quantification of the specific target is expressed as the ratio of target-specific antibody/actin. n = 4, *p = 0.02 for PERK, p = 0.01 for GCN2, and p = 0.04 for PKR. C, p-eIF2α is expressed as the ratio between anti-phospho antibody (Ser51) and anti-protein antibody; results were normalized to scrambled sequence control vector (SCR)-infected 3T3 cells (Scrambled; n = 4, ***p = 0.007, Student's t test). D, p-eIF2α is expressed as the ratio between anti-phospho antibody (Ser51) and anti-protein antibody; results were normalized to SCR-infected primary neuronal cells (n = 5–10, ***p = 0.03). Data are mean ± SEM.