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. 2014 Nov 19;34(47):15816–15831. doi: 10.1523/JNEUROSCI.1865-14.2014

Figure 2.

Figure 2.

Lineage-tracing experiments using Olig2Cre/+ knock-in mice. A, Schematic representation of the Olig2 lineage-tracing experiments. Progenitor cells of the ventral spinal cord that express Olig2 and all their progeny are marked by RFP expression in Olig2Cre/+;ROSA26-loxP-STOP-loxP-RFP embryos. Analyses of these embryos are presented in B–E. B, Triple immunostaining of Olig2 (green), RFP (red), and Prox1 (gray) in E8.5 mouse spinal cord. The brackets indicate the Olig2/RFP expression domain that is characterized by the absence of Prox1-expressing cells at this early stage. C, Triple immunostaining of Olig2 (green), Nkx6.1 (blue), and RFP (red) in E10.5 mouse spinal cord. The brackets indicate the p2 (Nkx6.1+ and Olig2−) and pMN (Nkx6.1+ and Olig2+) domains, as indicated. Note that RFP+ cells are detected into the p2 domain. D, E, Double immunostainings of Prox1 (green) and RFP (red) in E10.5 (D) or E13.5 (E) mouse spinal cords. The arrows indicate cells that coexpress Prox1 and RFP. Scale bars: 50 μm.