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. 2014 Nov 12;34(46):15297–15305. doi: 10.1523/JNEUROSCI.3061-14.2014

Figure 2.

Figure 2.

GnRH signaling is necessary for the establishment of vasopressin and RP3V TH sexual dimorphism. Vasopressin immunoreactivity in the LS of adult intact male (A), intact female (B), and wild-type (C) and GnRHR-KO (D) male mice GDX on P41–P45 and treated with a testosterone capsule for 14 d. E, Quantitative analyses (mean ± SEM) of the density of vasopressin fibers in the LS in intact wild-type male and female mice, and GDX+T wild-type and GnRHR-KO male mice. TH immunoreactivity in the RP3V of adult intact male (F), intact female (G), and wild-type (H) and GnRHR-KO (I) male mice GDX on P41–P45 and treated with a testosterone capsule for 14 d. J, Quantitative analyses (mean ± SEM) of the number of TH-immunoreactive neurons in the RP3V in wild-type intact male and female mice and GDX + T wild-type and GnRHR-KO male mice (n = 4–5/group). Scale bar, 100 μm. Groups were compared using one-way ANOVA with post hoc Student–Newman–Keuls comparisons. Bars labeled with different letters are significantly different from each other at either p < 0.05 or p < 0.01 (see text). LV, lateral ventricle; 3V, third ventricle.