Figure 6.

The reduction of the muscle contraction after inhibition of AChE and BChE is partially reversed by the block of α7 nAChR. A, Experimental protocol. The phrenic-diaphragm preparation was mounted in the organ bath. The muscle is indirectly stimulated by the nerve and the twitch was quantified with a tension sensor. The muscle was stimulated each 30 s by 4 stimulations at 2 Hz. B, Example of the representative traces recorded before and 20 min after incubation with neostigmine (3 μm). C, Amplitude of 6 TOFs obtained from in control condition, in atropine (0.1 μm), in MLA (10 nm), and in α7 nAChR KO mice. All values correspond to the mean of six successive TOFs in six different animals.