Figure 7.

Rescue of MG rat with specific AChE inhibitor and the effect of subsequent BChE inhibition. A, Experimental protocol. Rats were immunized with a peptide (sequence of main immunogenic region of rat muscle type AChR). EAMG was induced in 2 months. MG was tested by electromyography (EMG) recorded on leg of anesthetized rats. B, Examples of integral muscle action potential (imAP) trace in intact (left) and at EAMG (right) muscles. The first nerve stimulation triggered a trace (AP1, red solid line). After 200 nerve stimulations at 40 Hz, the trace (AP200) is shown as a blue dashed line. C, The ratio of the amplitudes of AP200 versus AP1 was used for the quantification. The graph presents the percentage of AP200 versus AP1 in a control rat and in an EAMG rat before and after AChE inhibition (injection of 5 μg/kg ambenonium, i.p.) and subsequent BChE inhibition (injection of 0.2 mg/kg bambuterol, i.p.) (n = 5, *p < 0.05, unpaired Student's t test).