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. 2014 Dec 3;34(49):16309–16319. doi: 10.1523/JNEUROSCI.4622-13.2014

Figure 1.

Figure 1.

Illustration of anatomical interactions between SRIF and NPY-expressing neurons within the hypothalamus (A, B) by immunofluorescence (C–K) and in situ hybridization (L–N). Brain slices were collected between −0.94 and −1.70 bregma. Schematic representation of specific areas within the hypothalamus, representative of the PeVN (A, enlarged inset, shaded areas in red) and ARC (B, enlarged inset, shaded area in red). Within the PeVN (C–H) we observed a number of SRIF-immunopositive neurons (red) in close apposition with NPY-GFP-immunopositive fibers (green; C–E). Further assessment using SV2 (gray; F and G; purple; H) revealed the synaptic interactions between SRIF and NPY-positive fibers (F–H; colocalization is indicated by white arrows). SRIF-positive immunoreactivity and NPY-GFP within the ARC (I–K) demonstrate punctuate SRIF expression, distributed among NPY-positive GFP neuronal cell bodies. In situ hybridization illustrates the proportion of Srif (black) and Npy (light blue) mRNA-expressing neurons within the ARC (L–N). These two populations of neurons were in close proximity. Scale bars: C–E, I–N, 100 μm; F–H, 10 μm. Inserts illustrate a magnified view of the figures. Representative images illustrate interactions verified across four animals.