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. 2014 Dec 10;34(50):16784–16795. doi: 10.1523/JNEUROSCI.1867-14.2014

Figure 2.

Figure 2.

CD8+ T-cell depletion decreases neuronal loss. A, Representative images of NeuN–TUNEL costaining of the infarcted area 3 d after pMCAO. Scale bar, 500 μm. B, Left, Analysis strategy for neuronal death: 300 × 300 μm regions (R1–R6) were chosen in the infarcted area as indicated. Right, In each region, the numbers of intact neurons (NeuN+ TUNEL−) were expressed as percentage value of all neurons (NeuN+). Data indicate the mean value of stainings from five IgG-treated mice (*p < 0.05 for day 1 vs day 7). C, Representative images of NeuN–TUNEL costaining of R1 1 and 7 days after pMCAO in IgG-treated mice and 7 d after pMCAO from CD8+ T-cell-depleted (anti-CD8) mouse. Scale bar, 50 μm. D, Intact (NeuN+ TUNEL−) and apoptotic (NeuN+ TUNEL+) neurons in R1 were counted 1, 3, and 7 d after pMCAO in IgG-treated mice and 7 d after pMCAO from anti-CD8 mice. Alive (white bar) and apoptotic (gray bar) cell numbers are shown as percentage of all neurons (n = 5 per group, *p < 0.05, #p < 0.05 for 7 d IgG vs 7 d anti-CD8). Data are expressed as mean ± SD.