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. 2014 Dec 10;34(50):16784–16795. doi: 10.1523/JNEUROSCI.1867-14.2014

Figure 4.

Figure 4.

CTLs but not NK cells are activated after brain invasion. A, Gating strategy for analysis of CD69 expression in CTLs. The dot plots on top indicate gating of CD8+ cells, and bottom plots show the assessment of CD69-expressing CD8+ cells. B, Gating strategy for analysis of B220 expression on NK1.1+ cells. The dot plots on top indicate gating of NK cells, and bottom plots show the assessment of B220-expressing NK cells. C, CD69, CD44, and CD62L mean fluorescence intensity (MFI, arbitrary units) of splenic and cerebral CTLs isolated 7 d after MCAO or sham operation (5 individual experiments with 5 pooled animals per group in each experiment; *p < 0.05). B220, NKp46, and CD11c (D) and Tbet and Eomes (E) MFI of splenic and brain-invading NK cells isolated 7 d after MCAO or sham operation (5 individual experiments with 5 pooled animals per group in each experiment). F, Ratio of in vitro IFN-γ-producing CTLs and NK cells isolated from spleen and brain 7 d after MCAO (4 individual experiments with 5 pooled animals per group in each experiment; *p < 0.05). Data in C–F are expressed as mean ± SD.