Figure 4.

Bre1a knockdown impairs the migration of neural precursor cells. Bre1a or control shRNA and GFP expression plasmids were microinjected into the lateral ventricle of E13.5 embryos and electroporated into the dorsal forebrain in utero. A, Three days after the electroporation, the embryos were fixed and coronal cryosections of the brains were immunostained for GFP. B, GFP⁺ cells in the VZ/SVZ, IMZ, or CP were counted. C, D, Twenty-four hours after the electroporation, coronal slices were made and cultured in serum-free media for 30 h. C, Time-lapse images of GFP⁺ cells in the CP. Arrowheads indicate migrating cells. D, Migration speed of GFP⁺ cells in the CP was quantified. Scale bars: A, 100 μm; C, 20 μm. Error bars indicate SEM, and n values are shown in columns. *p < 0.05 by one-way ANOVA followed by Dunnett's post hoc comparison (B) or by Student's t test (D).