Figure 1.
Morphological NMJ phenotypes of dAcsl mutants. A, B, Representative confocal images of NMJ4 from abdominal segments A3 to A6 costained with anti-HRP (green) and anti-CSP (magenta) in wild-type control (A) and dAcslKO/dAcsl05847 mutants (B). The NMJ terminals look similar across different abdominal segments of wild-type. In dAcsl mutants, however, the NMJs innervating the anterior segment muscles (i.e., A3) with shorter axons were overgrown with more satellite boutons, whereas those innervating the posterior segment muscles (i.e., A6) with longer axons were dystrophic. Scale bar, 10 μm. C, D, Quantitative results of the total bouton number (C) and satellite bouton number (D). n > 18 NMJs for all genotypes. ***p < 0.001 (Student's t test). Error bars indicate SEM.