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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Int J Biol Macromol. 2019 May 10;134:445–457. doi: 10.1016/j.ijbiomac.2019.05.060

Figure 1.

Figure 1

Representative UV-Vis spectra of (A) Fe(III)PPIX(Cl); (B) Mg(II)PPIX; (C) Mn(III)PPIX; (D) Sn(IV)PPIX as a function of the preparations steps used to eliminate the contribution of porphyrin aggregates. Initial PP concentrations were 2.6 μM (A), 4.6 μM (B), 4.1 μM (C) and 1.4 μM (D). For each PP the following spectra are represented: PP in aqueous solution (black); PP in the presence of HSA before dialysis: {HSA concentration = 6.8 μM (A), 8.8 μM (B), 10 μM (C) and 2.9 μM (D)} (red); the same sample after 60 hours dialysis (blue) and the dialyzed sample after 5 minutes centrifugation (green).