. Author manuscript; available in PMC: 2020 Aug 1.

Published in final edited form as: Int J Biol Macromol. 2019 May 10;134:445–457. doi: 10.1016/j.ijbiomac.2019.05.060

Table 4.

Fluorescence lifetime and corrected efficiency for FRET between Trp214 and each PP ligand^a.

Sample	⟨τ_D⟩ (ns)	⟨τ_DA⟩ (ns)	f_A	E_c(%)
HSA-PPIX	4.80	3.58	0.59 ± 0.08	0.43 ± 0.10
HSA-ZnPPIX	“	4.10	0.37± 0.16	0.39 ± 0.05
HSA-Hemin	“	3.06	0.53± 0.12	0.68 ± 0.16
HSA-Mg(II)PPIX	“	3.38	0.70 ± 0.14	0.42 ± 0.10
HSA-Mn(III)PPIX	“	4.10	0.50 ± 0.12	0.29 ± 0.04
HSA-Sn(IV)PPIX	“	3.88	0.96 ± 0.10	0.20 ± 0.03

The table reports the amplitude-weighted average lifetime of HSA (τ_D) and HSA-Fe(III)PPIX(Cl)/ Mg(II)PPIX/ Mn(III)PPIX/ Sn(IV)PPIX (τ_DA), the calculated values of energy transfer efficiency (E), the fractional labeling factor (f_A), the corrected energy transfer efficiency ^[47].