Table 2. Kinetic parameters for hydrolysis of GG and MG.
Experimental data were analysed using the allosteric kinetic model. A lower affinity for MG is expected owing to the higher estimated K 0.5 value.
| Kinetic parameters | GG | MG |
|---|---|---|
| V max (µmol min−1 per milligram of protein) | 3.60 ± 0.18 | 3.09 ± 0.66 |
| K 0.5 (mM) | 9.36 ± 0.69 | 84.18 ± 30.27 |
| h | 1.77 ± 0.20 | 1.29 ± 0.23 |
| R 2 | 0.974 | 0.990 |