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Evolution of B factors as a function of dose for the irradiation series of AtPhot2LOV2 crystals at cryogenic and room temperature. The evolution of the Wilson B factor (blue, 100 K; red, 293 K) illustrates global radiation damage and the evolution of the atomic B factor of Cys426 Sγ (green, 100 K; orange, 293 K) illustrates the specific radiation damage to the covalent bond Cys426–FMN that occurs concurrently with the time-dependent relaxation of the photoadduct.
