Table 1.
Surface area measurements
| VLP | Experimentally determined external surface areas (nm2) |
Calculated numbers of molecules |
||
|---|---|---|---|---|
| Envelope/matrix | Capsid | MA | CA | |
| 10a* | 53,000 | 27,000 | 4700 | 1600 |
| 10c | 45,000 | 18,000 | 3600 | 1100 |
| 1b | 46,000 | 17,000 | 3500 | 1000 |
| 1c | 48,000 | 17,000 | 4000 | 1000 |
| 1d | 47,000 | 16,000 | 3700 | 1000 |
| 1e | 52,000 | 19,000 | 4200 | 1200 |
| 1g* | 62,000 | 59,000 | 5100 | 3600 |
| 1k | 49,000 | 21,000 | 4300 | 1300 |
| 1m | 45,000 | 16,000 | 3400 | 900 |
| 2a | 48,000 | 18,000 | 3900 | 1100 |
| 3f | 52,000 | 17,000 | 4300 | 1000 |
| 3g* | 54,000 | 33,000 | 4200 | 2000 |
| 3i | 47,000 | 17,000 | 3900 | 1000 |
| 4a | 39,000 | 14,000 | 2800 | 900 |
| 4e | 46,000 | 16,000 | 3400 | 900 |
| 6c | 56,000 | 20,000 | 4500 | 1200 |
| 6e | 45,000 | 16,000 | 3400 | 1000 |
| 6b | 60,000 | 24,000 | 4900 | 1400 |
| 1n | 52,000 | 20,000 | 4400 | 1200 |
| 2b | 48,000 | 19,000 | 3700 | 1200 |
| 2f | 50,000 | 21,000 | 4000 | 1300 |
| 3j | 51,000 | 19,000 | 4000 | 1200 |
| 4k | 45,000 | 17,000 | 3600 | 1000 |
| 7g | 53,000 | 23,000 | 4200 | 1400 |
| 9e | 45,000 | 18,000 | 3300 | 1100 |
| Mean | 49,000 | 21,000 | 4000 | 1300 |
| Stddev | 5200 | 9000 | 530 | 540 |
The surface areas of each segmented envelope/matrix and capsid shell were measured and presented in the same order as they appear in Figure 2. Assuming each is a hexagonal net, and taking lattice spacings from the literature (see the text), the numbers of MA and CA monomers involved were estimated.
(*)
Asterisks mark VLPs with multiple capsids, where the entire capsid surface area was summed.