Fig. 5.

Quantifying recombinagenic and mutagenic frequencies of gene induction and regulation with allele-specific qPCR assays of the gamma-globin genomic locus. Allele-specific qPCR assays are used to (a) quantify the recombinagenic and mutagenic frequency of recombinagenic donor DNA or recombinagenic donor DNA co-transfected with mutagenic triplex-forming molecules, in the gene induction and regulation of the gammaglobin genomic locus by correlating the relative qPCR amplification values with modification frequencies, (b) with varying frequencies of gene induction and regulation attributed to the particular molecules used in the treatment