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. Author manuscript; available in PMC: 2019 Jul 3.
Published in final edited form as: Methods Mol Biol. 2015;1239:39–73. doi: 10.1007/978-1-4939-1862-1_4

Fig. 6.

Fig. 6

Quantifying recombinagenic and mutagenic frequencies of gene disruption with single-cell cloning assays of the CCR5 genomic locus. Single-cell cloning assays are used to (a) quantify the recombinagenic and mutagenic frequency of recombinagenic donor DNA alone or recombinagenic donor DNA co-transfected with mutagenic triplex-forming molecules, in the gene disruption of the CCR5 genomic locus by expanding each single cell to a population of clones sufficient in number to enable allele-specific PCR-based verification and calculation of modification frequency, (b) with sequencing chromatographic mixed peaks indicating a heterozygous clone (wild type sequence being TGTCAT; modified sequence being CTGAGG)