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. 2014 Mar 5;34(10):3756–3766. doi: 10.1523/JNEUROSCI.5123-13.2014

Figure 2.

Figure 2.

De novo expression and maturation of the GPR54 system in female mice. A, Genetic strategy to analyze the embryonic development of GPR54 neurons. GPR54 neurons in GPIC/eR26-τGFP double-heterozygous mice express τGFP from the recombined ROSA26 allele. B–G, Double immunofluorescence for GnRH (red) and τGFP (green) of a sagittal section through the head of a GPIC/eR26-τGFP female at E12.5 (B, E), E13.5 (C, F), and E16.5 (D, G). Note that GPR54 expression starts at E13.5 and is restricted to GnRH neurons throughout embryonic development. H, Quantification of GnRH/GPR54 neurons throughout embryonic maturation. ***p < 0.001, **p < 0.01. Nuclei were stained with Hoechst 33258 (blue). OE, Olfactory epithelium; fb, forebrain; LV, lateral ventricle; CB, cribriform plate; ME, median eminence. Scale bars: B–D, 200 μm; E–G, 50 μm.