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. 2014 Apr 9;34(15):5322–5334. doi: 10.1523/JNEUROSCI.4703-13.2014

Figure 1.

Figure 1.

Representative photomicrographs of GLP-1R expression in HEK293 cells stably expressing human GLP-1Rs (A, B), HEK293T cells that do not express GLP-1Rs (C, D), PC12 cells expressing rat GLP-1Rs (E, F), and their corresponding negative controls of the secondary antibody (B, D, F), as well as the superficial spinal dorsal horn (laminae I-III; G), cortex (H), hippocampus (I), dorsal root ganglia (J), pancreatic islets (K), and skeletal muscle (L) from 6 normal rats. Tissues were doubly labeled with the GLP-1R and GAPDH antibodies. DAPI staining was also used to determine cell nuclei. Representative photomicrographs for the preventive effect of the GLP-1R antigenic peptide EQ14 on GLP-1R immunoreactive fluorescence staining in the spinal cords from 6 spinal nerve ligation-induced neuropathic rats. M, Specific fluorescence staining with the GLP-1R antibody alone. N, Fluorescence staining with the GLP-1R antibody in the presence of EQ14. O, Fluorescence staining with EQ14 alone. P, Negative control of the secondary antibody. Scale bars: A–F, 150 μm; G–L, 100 μm; M–P, 300 μm.