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. 2019 Jul 3;14(7):e0219181. doi: 10.1371/journal.pone.0219181

Fig 2. P. gingivalis supernatant impairs the osteogenic potential of PDLSC.

Fig 2

(A) IL-1 β, IL-6 and IL-8 production was measured using ELISA after PDLSCs were treated with P. gingivalis (P.g.) supernatant’s dilutions (1:500 and 1:50), or untreated cells as control simultaneously for 24 h. IL-1 β, IL-6 and IL-8 is expressed in pg/ml ( ± standard deviation). (B) Western blot analysis showed the protein expression of TLR2, TLR4 and GAPDH was used as the internal control. (C) Confluent PDLSC were stained with Alizarin Red after 21 days of cultivation in osteogenic medium containing P. gingivalis supernatant’s dilutions (1:500 and 1:50) and Alizarin Red was then extracted and measured for light absorbance at 405 nm. *p < 0.05 as determined by unpaired two-tailed t tests. Data from three biologically independent replicates.