Fig. 5.

Treatment with 2AP during the indeterminate stage reduced cardiac inflammation in chronic T. cruzi infected mice (n=10). (A) Immunoblot analysis demonstrated a significant decrease in the level of TNFα in the hearts of infected mice treated with 2AP compared to infected untreated mice at 120 DPI. (B) Fold changes in the protein levels of TNFα were normalized to GDI expression and represented as the bar graph. (C) qPCR analysis demonstrates a significant decrease in the mRNA levels of TNF-A in the hearts of infected mice treated with 2AP compared to infected untreated mice at 120 DPI. Statistical significance compared to uninfected untreated mice and infected untreated mice are represented by ^*p≤0.05, ^***p≤0.001, and ^#p≤0.05 or ^##p≤0.01, respectively.