Fig. 3.

Effect of TY52156 on iNOS protein expression and NO production in macrophages. Mouse peritoneal macrophages were treated with the indicated concentrations of TY52156 for 1 h, and then treated with LPS 100 ng/mL for 24 or 48 h. Western blotting was conducted on cell lysates. The data shown in (A) are representative of three independent experiments. Relative protein levels of iNOS versus β-actin are presented as histograms (B). LPS-induced productions of nitrite (C) was measured. TY52156 inhibited nitrite production in a concentration-dependent manner (C). Results are the means ± standard deviation of three independent experiments. Statistically significant at ^###p<0.001 level vs. the vehicle-treated macrophages, and at ^**p<0.01 and ^***p<0.001 vs. the LPS-treated macrophages.