Fig. 4.

Effect of TY52156 on the protein expression of COX-2 in macrophages. Mouse peritoneal macrophages were treated with the indicated concentrations of TY52156 for 1 h, and then treated with LPS 100 ng/mL for 24 h. Western blotting was conducted on cell lysates. The data shown in (A) are representative of three independent experiments. Relative protein levels of COX-2 versus β-actin are presented as histograms (B). Results are the means ± standard deviation of three independent experiments. Statistically significant at ^###p<0.001 level vs. the vehicle-treated macrophages, and at ^*p<0.05 vs. the LPS-treated macrophages.