Fig. 5.

Effect of TY52156 on the expression of IL-1β, IL-6, and TNF-α in macrophages. (A) Mouse peritoneal macrophages were treated with the indicated concentrations of TY52156 for 1 h, and then treated with vehicle or LPS 10 ng/mL for 5 h, and RT-PCR was performed to detect the expression of the pro-inflammatory genes, IL-1β, IL-6, and TNF-α. The data shown are representatives of three independent experiments. (B–D) Histograms of quantitated mRNA expression of IL-1β, IL-6, and TNF-α. Results are the means ± standard deviation of three independent experiments. Statistically significant at ^###p<0.001 level vs. the vehicle-treated macrophages, and at ^*p<0.05, ^**p<0.01 and ^***p<0.001 vs. the LPS-treated macrophages.