Fig. 6.

Effect of TY52156 on IL-1β protein expression and IL-1β production in macrophages. Mouse peritoneal macrophages were treated with the indicated concentrations of TY52156 for 1 h, and then treated with LPS 100 ng/mL for 24 h. Western blotting was conducted on cell lysates. The data shown in (A) are representatives of three independent experiments. Relative protein levels of IL-1β versus β-actin are presented as histograms (B, C). LPS-induced production of IL-1β in the media was measured by ELISA (D). Results are the means ± standard deviation of three independent experiments. Statistically significant at ^##p<0.01 and ^###p<0.001 vs. the vehicle-treated macrophages, and at ^*p<0.05 and ^**p<0.01 vs. the LPS-treated macrophages.